Cloud-clone
SKU:LMC243Hu
Magnetic Luminex Assay Kit for Topoisomerase I (TOP1) ,etc.
Magnetic Luminex Assay Kit for Topoisomerase I (TOP1) ,etc.
Couldn't load pickup availability
Product No.
LMC243Hu
Organism Species
Homo sapiens (Human).
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.01-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.003 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Topoisomerase I (TOP1) ,etc..
No significant cross-reactivity or interference between Topoisomerase I (TOP1) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Topoisomerase I (TOP1) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Topoisomerase I (TOP1) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-98 | 83 |
| EDTA plasma(n=5) | 89-97 | 94 |
| heparin plasma(n=5) | 84-104 | 90 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Topoisomerase I (TOP1) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Topoisomerase I (TOP1) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Topoisomerase I (TOP1) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-103% | 94-102% | 93-104% | 98-105% |
| EDTA plasma(n=5) | 78-95% | 88-96% | 96-104% | 78-89% |
| heparin plasma(n=5) | 79-104% | 80-94% | 85-93% | 90-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:TOP1) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Electronic Journal of Biotechnology | Comparing the expression of human DNA topoisomerase I in KM71H and X33 strains of Pichia pastoris science:S0717345816000233 |
| Journal of Experimental & Clinical Cancer Research | Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase I and topoisomerase II pubmed:27852319 |
| Tikrit Journal of Pure Science | Partial purification of topoisomerase IB from serum of diabetic patients and study it's kinetic properties and molecular weight |
| EurAsian Journal of BioSciences | Study of topoisomerase I (Topo I) level and some biochemical parameters in diabetic patients |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC243Hu01 | Recombinant Topoisomerase I (TOP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC243Hu01 | Polyclonal Antibody to Topoisomerase I (TOP1) | WB; IHC; ICC; IP. |
| MAC243Hu22 | Monoclonal Antibody to Topoisomerase I (TOP1) | WB; IHC; ICC; IP. |
| SEC243Hu | ELISA Kit for Topoisomerase I (TOP1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC243Hu | Magnetic Luminex Assay Kit for Topoisomerase I (TOP1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
Share
