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SKU:LMG945Hu
Magnetic Luminex Assay Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc.
Magnetic Luminex Assay Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc.
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Product No.
LMG945Hu
Organism Species
Homo sapiens (Human).
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.05-50ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.017 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc..
No significant cross-reactivity or interference between Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 82-101 | 86 |
| heparin plasma(n=5) | 84-91 | 88 |
| sodium citrate plasma(n=5) | 89-97 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 91-101% | 86-101% | 92-99% |
| EDTA plasma(n=5) | 79-102% | 87-104% | 95-104% | 89-96% |
| heparin plasma(n=5) | 91-104% | 78-91% | 82-97% | 90-98% |
| sodium citrate plasma(n=5) | 96-103% | 97-105% | 92-104% | 88-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:UCHL1) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| J Neurol Sci | Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions PubMed: 26232084 |
| Journal of the Neurological Sciences | Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome Pubmed:26810533 |
| Ulutas Medical Journal | Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy mnstemps:135 |
| Scientific Reports | Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes pubmed:28667290 |
| Intensive Care Medicine | Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability Pubmed:29523900 |
| Earth and Environmental Science | Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury article:10.1088 |
| Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage ISBN:978-952-03-0750-9 | |
| Journal of Critical Care | Association of neuronal repair biomarkers with delirium among survivors of critical illness Pubmed: 31896448 |
| Brain Sciences | BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma Pubmed: 32987792 |
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