FineTest
SKU:FNab00001
anti- 14-3-3 antibody
anti- 14-3-3 antibody
Couldn't load pickup availability
Introducing the ANTI-14-3-3 ANTIBODY, a highly specific and sensitive tool for detecting endogenous levels of total 14-3-3 protein. This antibody is available in a convenient size of 100μg and is capable of detecting all known isoforms of mammalian 14-3-3 proteins (β/α, γ, ε, η, ζ/δ, θ/τ and σ).
The 14-3-3 proteins play a pivotal role in regulating diverse cellular processes, making them a focal point in cell signaling research. Our ANTI-14-3-3 ANTIBODY provides a reliable tool for the accurate recognition of endogenous levels of total 14-3-3 protein, enabling you to delve deeper into their functional significance.
Whether you're exploring cell cycle regulation, apoptosis, signal transduction pathways, or protein-protein interactions, the ANTI-14-3-3 ANTIBODY provides the versatility and precision needed for impactful discoveries.
With its high specificity and sensitivity, this antibody is an essential component of any researcher's toolkit, enabling the investigation of cellular signaling pathways and disease states.
-
Product Name
14-3-3 antibody
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
BACKGROUND
14-3-3 theta(also known as YWHAQ, or 14-3-3 tau) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms(gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo-and hetero-dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 theta.
IMMUNOGEN INFORMATION
Immunogen
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Synonyms
14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 1C5, HS1, Protein HS1, YWHAQ
Observed MW
28 kDa
APPLICATION
Tested Application
ELISA, IF, IP, IHC, WB
Recommended Dilution
WB: 1:500-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
UNIPROT INFORMATION
UniProt ID
IMAGES
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using FNab00001(14-3-3 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using FNab00001(14-3-3 Antibody) at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti 14-3-3(IP:FNab00001, 5μg; Detection:FNab00001 1:500)with mouse lung tissue lysate 4000μg.
Hela cell were subjected to SDS PAGE followed by western blot with FNab00001(14-3-3 Antibody) at dilution of 1:1000
