anti- AP2A2 antibody

Introducing our Anti-AP2A2 Antibody, a powerful and versatile tool designed to empower your research into the intricate world of intracellular trafficking and vesicle formation. AP2A2, also known as Adaptor Protein Complex 2 Alpha 2 Subunit, is a fundamental component of the adaptor protein complex, playing a pivotal role in the sorting and transportation of cargo within cells. Our meticulously crafted antibody, available in a 100µg size, offers exceptional specificity and sensitivity, ensuring the precision and reliability necessary for your research endeavors.

With 100µg of this high-quality antibody at your disposal, you have the flexibility to conduct a wide range of experiments and assays. Whether you're investigating AP2A2's roles in clathrin-mediated endocytosis, cargo selection, or exploring its implications in cellular function and disease pathways, this antibody equips you with the precision required for impactful scientific discoveries.

Our Anti-AP2A2 Antibody has undergone rigorous validation to ensure its reliability and reproducibility. You can trust in the consistency of your results, enabling you to draw accurate conclusions and make significant strides in your research endeavors.

AP2A2's multifaceted functions in intracellular trafficking demand an antibody of exceptional quality, and our product is precisely engineered to meet these demands. Whether you are an experienced cell biologist or a newcomer to the field, our Anti-AP2A2 Antibody promises to be an invaluable asset in your laboratory. Trust in its quality to drive your scientific discoveries forward, one experiment at a time, as you unravel the complexities of intracellular transport mechanisms.

Product Name

AP2A2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Component of the adaptor protein complex 2(AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin(clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein(AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV](Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif(By similarity).

IMMUNOGEN INFORMATION

Immunogen

adaptor-related protein complex 2, alpha 2 subunit

Synonyms

ADTAB, CLAPA2, HIP9, HYPJ, KIAA0899

Observed MW

104 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:200-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

O94973

IMAGES