anti- APEX1 antibody

Uncover the mysteries of DNA repair and oxidative stress response with our Anti-APEX1 Antibody. APEX1, or Apurinic/Apyrimidinic Endodeoxyribonuclease 1, is a key enzyme essential for DNA base excision repair and redox regulation. Our meticulously crafted antibody, available in a 100µg size, provides exceptional specificity and sensitivity, ensuring the precision and reliability required for your research endeavors.

With 100µg of this high-quality antibody at your disposal, you have the flexibility to conduct a wide range of experiments. Whether you're exploring APEX1's roles in DNA damage repair, deciphering its interactions within DNA repair complexes, or delving into its implications in cancer and aging, this antibody empowers you with the precision needed for impactful scientific discoveries.

Our Anti-APEX1 Antibody has undergone rigorous validation to ensure its reliability and reproducibility. Trust in the consistency of your results, enabling you to draw accurate conclusions and make significant strides in your research endeavors, particularly in the fields of genomics, cancer research, and DNA repair mechanisms.

Unleash the power of DNA repair and redox regulation with confidence. Whether you are an experienced molecular biologist or just beginning your scientific journey, our Anti-APEX1 Antibody promises to be an invaluable asset in your laboratory, driving your research forward as you explore the intricate world of DNA maintenance and cellular defense mechanisms.

Product Name

APEX1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic(AP) endodeoxyribonuclease in the DNA base excision repair(BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions(such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate(dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination(CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone(PTH) expression by binding to negative calcium response elements(nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant(CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.

IMMUNOGEN INFORMATION

Immunogen

APEX nuclease(multifunctional DNA repair enzyme) 1

Synonyms

APE, APE1, APEX, APX, HAP1, REF1

Observed MW

36 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:100; IF: 1:50 - 1:100

UNIPROT INFORMATION

UniProt ID

P27695

IMAGES