FineTest
SKU:FNab00528
anti- ARF1 antibody
anti- ARF1 antibody
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Explore the intricacies of intracellular vesicle trafficking and membrane dynamics with our Anti-ARF1 Antibody, available in a convenient 100µg size. ADP-Ribosylation Factor 1 (ARF1) is a small GTPase known for its crucial role in regulating vesicle budding and cargo sorting.
This antibody offers exceptional sensitivity and specificity, ensuring reliable and reproducible results in various applications, from Western blotting to immunofluorescence. Whether you're investigating ARF1's involvement in Golgi apparatus organization or its role in membrane remodeling, our antibody is your trusted research companion.
The 100µg size offers cost-effective flexibility, accommodating both small-scale experiments and larger research initiatives. Its consistent quality and purity make it an ideal choice for researchers across diverse disciplines, from cell biology to biochemistry.
Choose our Anti-ARF1 Antibody to propel your research forward and gain fresh insights into ARF1-related processes. Rely on its performance and join the community of scientists who trust our products to drive scientific discovery.
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Product Name
ARF1 antibody
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
BACKGROUND
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor(AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression(LTD).
IMMUNOGEN INFORMATION
Immunogen
ADP-ribosylation factor 1
Observed MW
21 kDa
APPLICATION
Tested Application
ELISA, WB, IHC
Recommended Dilution
WB: 1:500-1:2000; IHC: 1:20-1:200
UNIPROT INFORMATION
UniProt ID
IMAGES
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Immunohistochemistry of paraffin-embedded human small intestine tissue slide using FNab00528(ARF1 Antibody) at dilution of 1:50 -
HeLa cells were subjected to SDS PAGE followed by western blot with FNab00528(ARF1 antibody) at dilution of 1:500
