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SKU:FNab00952

anti- BrdU antibody

anti- BrdU antibody

Regular price $330.00 USD
Regular price Sale price $330.00 USD
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Investigate BrdU (5-Bromo-2'-deoxyuridine) incorporation with our Anti-BrdU Antibody, available in a 100µg size. This high-quality antibody is your versatile tool for detecting and quantifying BrdU-labeled DNA in cellular proliferation and DNA synthesis assays.

Engineered for precision and reliability, this antibody ensures exceptional sensitivity in applications such as immunohistochemistry, ELISA, and flow cytometry. Whether you're studying cell proliferation, tissue regeneration, or DNA labeling, this antibody enhances your research capabilities.

BrdU is a thymidine analog widely used to label newly synthesized DNA during cell proliferation. It is a valuable tool for assessing cell division, tissue growth, and DNA replication in various research areas.

With our Anti-BrdU Antibody, you can accurately assess BrdU incorporation, track cell cycle progression, and potentially advance research in developmental biology and regenerative medicine. Elevate your research in DNA synthesis and cell proliferation with this precision antibody.

Product Name

BrdU antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2a

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Bromodeoxyuridine(5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells(during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

IMMUNOGEN INFORMATION

Immunogen

compound

Synonyms

5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR

APPLICATION

Tested Application

ELISA, IF, IHC

Recommended Dilution

IHC: 1:200-1:1000; IF: 1:50-1:500

IMAGES

 

Immunohistochemistry of paraffin-embedded mouse spleen tissue slide(with BrdU feeding) using FNab00952(BrDu Antibody) at dilution of 1:400. Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using FNab00952 ( BrdU Antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

 

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