anti- CARM1 antibody

Our Anti-CARM1 Antibody, available in a 100µg vial, is a valuable research tool for scientists investigating the functions of CARM1 (Coactivator-Associated Arginine Methyltransferase 1) in gene regulation, transcriptional activation, and epigenetic modifications.

CARM1 is a protein methyltransferase that plays a crucial role in the methylation of histone proteins and non-histone substrates, thereby influencing gene expression and various cellular processes. This antibody is meticulously designed to target CARM1 with high specificity, ensuring accurate and reproducible results in your experiments. It is compatible with various applications, including Western blotting and immunoprecipitation, offering versatility in your research.

With the 100µg vial size, you'll have an ample supply of this high-quality antibody for multiple experiments, ensuring cost-effectiveness and consistent results. Dive into the intricate world of CARM1 and its significance in epigenetics and gene regulation with confidence, knowing you have a trusted research partner.

Product Name

CARM1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTMsites in cells themselves.

IMMUNOGEN INFORMATION

Immunogen

coactivator-associated arginine methyltransferase 1

Synonyms

CARM1, PRMT4

Observed MW

63-66 kDa, 55 kDa

APPLICATION

Tested Application

ELISA, IHC, WB, IF

Recommended Dilution

WB: 1:1000-1:5000; IHC: 1:50-1:500; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q86X55

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