1 1

FineTest

FNab01289

anti- Caspase 3 antibody

anti- Caspase 3 antibody

$290.00 USD
$290.00 USD
Sale Sold out
Shipping calculated at checkout.
size

Our Anti-Caspase 3 Antibody (100µg) is an indispensable tool for researchers investigating apoptosis, a fundamental process in cell biology. Caspase 3, a key executioner caspase, plays a central role in orchestrating cell death. This antibody, designed to target Caspase 3, guarantees precise and reproducible results in your experiments.

With 100µg of this antibody at your disposal, you'll have an ample supply to conduct multiple experiments, reducing the need for frequent reordering. Whether you're conducting Western blots, immunohistochemistry, or flow cytometry, this antibody consistently delivers high-quality performance.

The specificity and sensitivity of this antibody are backed by advanced production techniques and rigorous quality control. Its reliability makes it an invaluable asset for projects aimed at deciphering the mechanisms of apoptosis and cell death.

Advance your apoptosis research confidently with our Anti-Caspase 3 Antibody. By utilizing this antibody, you contribute to the ongoing exploration of the intricate pathways governing cell fate.

Product Name

Caspase 3 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. The antibody can recognize p17 and p32 of Caspase 3.

IMMUNOGEN INFORMATION

Immunogen

caspase 3, apoptosis-related cysteine peptidase

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

Observed MW

32-35 kDa

APPLICATION

Tested Application

ELISA, IHC, WB,IF,IP

Recommended Dilution

WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:500; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P42574

IMAGES

 

Immunohistochemistry of paraffin-embedded human spleen using FNab01289(CASP3 antibody) at dilution of 1:50
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed NIH/3T3 cells using FNab01289 (Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
IP result of anti-Caspase 3(IP:FNab01289, 4ug; Detection:FNab01289 1:300) with NIH/3T3 cells lysate 3440 ug
HeLa cells were subjected to SDS PAGE followed by western blot with FNab01289(CASP3 antibody) at dilution of 1:1000

 

View full details