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FNab01294

anti- Caspase 8 antibody

anti- Caspase 8 antibody

$310.00 USD
$310.00 USD
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Our Anti-Caspase 8 Antibody (Monoclonal, 100µg) is a specialized tool for researchers studying apoptosis and immune signaling pathways. Caspase 8, a key initiator caspase, is central to cell death triggered by death receptors. This monoclonal antibody is designed to specifically target Caspase 8, ensuring precision in your experiments.

The 100µg size provides an ample amount of antibody for multiple experiments, reducing the need for frequent restocking. Whether you're conducting Western blots, immunohistochemistry, or flow cytometry, this antibody consistently delivers high-quality results.

The monoclonal nature of this antibody ensures exceptional specificity and sensitivity, making it a preferred choice for discerning researchers. Its ability to recognize a single epitope with high affinity allows for accurate detection and quantification of Caspase 8.

Advance your research on apoptosis and immune signaling with confidence using our Anti-Caspase 8 Antibody. Its reliability and precision will empower you to uncover critical insights into these vital cellular processes and their implications in biology and disease.

Product Name

Caspase 8 antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816). It may control diabetic embryopathy-associated apoptosis via Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro-and cleaved-caspase 8.

IMMUNOGEN INFORMATION

Immunogen

caspase 8, apoptosis-related cysteine peptidase

Synonyms

Apoptotic cysteine protease, Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5

Observed MW

50-55 kDa, 25-32 kDa

APPLICATION

Tested Application

ELISA, IHC, WB,IF,IP

Recommended Dilution

WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:100-1:400; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

Q14790

IMAGES

 

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab01294(Caspase 8 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab01294 ( Caspase 8 Antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
IP Result of anti-Caspase 8 (IP:FNab01294, 5ug; Detection:FNab01294 1:500) with HepG2 cells lysate 2400ug.
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab01294(CASP8 antibody) at dilution of 1:1000

 

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