anti- CLP1 antibody

Uncover the mysteries of RNA processing and splicing with our Anti-CLP1 Antibody, available in a 100µg size. CLP1 (Cleavage and Polyadenylation Factor I Subunit 1) is an essential component of the cleavage and polyadenylation complex, playing a pivotal role in pre-mRNA 3' end processing.

This meticulously designed antibody offers unparalleled specificity and sensitivity, ensuring precise detection of CLP1 protein in your experiments. Researchers investigating RNA metabolism, gene expression, or related areas will appreciate the accuracy and reliability of this antibody.

Our high-quality Anti-CLP1 Antibody undergoes stringent quality control, guaranteeing consistent and reproducible results. Dive into the forefront of RNA biology research and gain valuable insights into the role of CLP1 in mRNA processing and gene regulation.

Product Name

CLP1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA(dsRNA), single-stranded RNA(ssRNA), double-stranded DNA(dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA(PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development(PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II(CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs(siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex(RISC). However, endogenous siRNAs and microRNAs(miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

IMMUNOGEN INFORMATION

Immunogen

CLP1, cleavage and polyadenylation factor I subunit, homolog

Synonyms

HEAB

Observed MW

48 kDa

APPLICATION

Tested Application

ELISA, WB

Recommended Dilution

WB: 1:500-1:2000

UNIPROT INFORMATION

UniProt ID

Q92989

IMAGES

mouse testis tissue were subjected to SDS PAGE followed by western blot with FNab01770(CLP1 antibody) at dilution of 1:600