anti- CPSF3 antibody

Dive into the fascinating world of mRNA processing and gene expression with our Anti-CPSF3 Antibody, available in a convenient 100µg size. Cleavage and Polyadenylation Specificity Factor 3 (CPSF3) is a crucial component of the pre-mRNA 3' end processing complex, essential for mRNA maturation and gene regulation.

This antibody is rigorously validated for specificity and sensitivity, making it suitable for various applications, including Western blotting, chromatin immunoprecipitation, and RNA immunoprecipitation. Whether you are studying RNA processing, polyadenylation, or transcriptional control, our Anti-CPSF3 Antibody will provide you with precise and reliable results.

Trust in this antibody's accuracy and reliability as you advance your research in molecular biology, RNA biology, and related fields. Uncover the molecular mechanisms governing mRNA maturation and post-transcriptional regulation with the assistance of this high-quality antibody.

Product Name

CPSF3 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Component of the cleavage and polyadenylation specificity factor(CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product(DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop(SL) and 5' phosphate on the downstream cleavage product(DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product(DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.

IMMUNOGEN INFORMATION

Immunogen

cleavage and polyadenylation specific factor 3, 73kDa

Synonyms

CPSF73

Observed MW

77kd

APPLICATION

Tested Application

ELISA, WB

Recommended Dilution

WB: 1:500-1:2000

UNIPROT INFORMATION

UniProt ID

Q9UKF6

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