anti- CTCF antibody

The anti-CTCF antibody, available in a 100µg size, is an essential tool for researchers studying the role of CCCTC-binding factor (CTCF) in chromatin organization and gene regulation. CTCF is a highly conserved transcription factor known for its involvement in genome architecture and epigenetic regulation.

This polyclonal antibody is meticulously designed to target CTCF with remarkable specificity and sensitivity, making it suitable for various applications, including chromatin immunoprecipitation (ChIP), immunofluorescence, and electrophoretic mobility shift assays (EMSA).

Our anti-CTCF antibody is sourced from high-quality materials and undergoes rigorous validation to ensure reliable and reproducible results. It is a trusted choice for researchers exploring the intricate mechanisms of gene expression, chromatin looping, and insulator function.

The 100µg size of this antibody provides researchers with an ample supply for multiple experiments, and it is compatible with various sample types, including cell lysates and chromatin extracts. Whether investigating chromatin structure, enhancer-promoter interactions, or epigenetic modifications, this antibody is a valuable asset for your research.

Advance your scientific investigations in genome regulation and epigenetics with confidence using the anti-CTCF antibody, backed by our commitment to excellence in molecular biology and chromatin research.

Product Name

CTCF antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

IMMUNOGEN INFORMATION

Immunogen

CCCTC-binding factor (zinc finger protein)

Observed MW

135 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:1000 - 1:2000; IHC: 1:50 - 1:200; ChIP : 1:50 - 1:200

UNIPROT INFORMATION

UniProt ID

P49711

IMAGES