anti- CTR9 antibody

The anti-CTR9 antibody, available in a 100µg size, is a valuable tool for researchers delving into the functions of CTR9, a component of the Polymerase-associated factor 1 (PAF1) complex, in various cellular processes, particularly its roles in transcriptional regulation and RNA polymerase II function.

This polyclonal antibody is meticulously designed to target CTR9 with exceptional specificity and sensitivity, making it ideal for a wide range of applications, including chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence.

Our anti-CTR9 antibody is sourced from high-quality materials and undergoes rigorous validation to ensure reliable and reproducible results. It is a trusted choice for researchers exploring the molecular mechanisms of transcriptional regulation, gene expression, and chromatin dynamics.

The 100µg size of this antibody provides researchers with an ample supply for multiple experiments, and it is compatible with various sample types, including cultured cells and tissue sections. Whether investigating gene transcription, RNA polymerase function, or epigenetic modifications, this antibody is an invaluable resource for your research.

Product Name

CTR9 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Component of the PAF1 complex(PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'-and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4'(H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B(H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono-and trimethylation on histone H3 'Lys-4'(H3K4me3) and dimethylation on histone H3 'Lys-79'(H3K4me3). Required for Hox gene transcription. Required for the trimethylation of histone H3 'Lys-4'(H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3(By similarity).

IMMUNOGEN INFORMATION

Immunogen

Ctr9, Paf1/RNA polymerase II complex component, homolog

Synonyms

KIAA0155, SH2BP1

Observed MW

130-150kd

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q6PD62

IMAGES