anti- CUL4B antibody

The anti-CUL4B antibody, available in a 100µg size, is an essential tool for researchers studying the functions of Cullin-4B (CUL4B) in various cellular processes, particularly its roles in ubiquitin ligase complexes and protein degradation pathways.

This polyclonal antibody is meticulously designed to target CUL4B with exceptional specificity and sensitivity, making it suitable for various applications, including Western blotting, immunohistochemistry, and co-immunoprecipitation assays.

Our anti-CUL4B antibody is sourced from high-quality materials and undergoes rigorous validation to ensure reliable and reproducible results. It is a trusted choice for researchers investigating the molecular mechanisms of protein turnover, cell cycle regulation, and DNA repair.

The 100µg size of this antibody provides researchers with an ample supply for multiple experiments, and it is compatible with various sample types, including cell lysates and tissue sections. Whether studying cancer biology, DNA damage response, or protein ubiquitination, this antibody is an invaluable resource for your research.

Product Name

CUL4B antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin(mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.

IMMUNOGEN INFORMATION

Immunogen

cullin 4B

Synonyms

KIAA0695

Observed MW

102 kDa

APPLICATION

Tested Application

ELISA, WB, IF, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q13620

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