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FNab02122

anti- Cyclin B1 antibody

anti- Cyclin B1 antibody

$290.00 USD
$290.00 USD
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Our Anti-Cyclin B1 Antibody, available in a 100µg size, is an invaluable tool for researchers investigating cell cycle regulation and mitosis. Cyclin B1, a member of the Cyclin B family, is a central player in controlling the G2 to M phase transition in the cell cycle.

Understanding the functions and interactions of Cyclin B1 is essential for unraveling its contributions to normal cell division and genome stability. Our antibody has been meticulously validated for specificity and sensitivity, ensuring reliable results in your experiments.

Its compact size allows for versatility in applications, whether you're conducting flow cytometry, immunohistochemistry, or in vitro cell cycle synchronization studies. Trust in the quality of our Anti-Cyclin B1 Antibody to advance your scientific investigations and contribute to advancements in cell biology and mitotic research.

Product Name

Cyclin B1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor(MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1.

IMMUNOGEN INFORMATION

Immunogen

cyclin B1

Synonyms

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

Observed MW

55 kDa

APPLICATION

Tested Application

ELISA, WB, IF, IHC, IP

Recommended Dilution

WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P14635

IMAGES

 

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using FNab02122(Cyclin B1 Antibody) at dilution of 1:50. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HeLa cells using FNab02122( Cyclin B1 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
IP Result of anti-Cyclin B1 (IP:FNab02122, 4ug; Detection:FNab02122 1:500) with HeLa cells lysate 1200ug.
HeLa cells were subjected to SDS PAGE followed by western blot with FNab02122(CCNB1 antibody) at dilution of 1:500

 

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