anti- DDB1 antibody

Our Anti-DDB1 (DNA Damage-Binding Protein 1) Antibody, available in a 100µg size as a monoclonal antibody, is a specialized tool for researchers investigating the role of DDB1 in DNA repair, cell cycle regulation, and protein degradation pathways. DDB1 is a scaffold protein that interacts with various partners and is involved in multiple cellular processes.

Accurate detection of DDB1 is crucial for understanding its functions and its impact on genome stability, cell division, and proteostasis. This monoclonal antibody has undergone rigorous validation for specificity and sensitivity, ensuring reliable results in applications such as immunohistochemistry and co-immunoprecipitation.

With its 100µg size, this antibody provides ample material for multiple experiments, allowing you to explore DDB1 expression and interactions in different cellular contexts and under various experimental conditions. Trust in the quality of our Anti-DDB1 Antibody (Monoclonal) to advance your research and deepen your insights into the multifaceted roles played by DDB1 in cellular biology.

Product Name

DDB1 antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2a

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex(the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway(the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers(CPD), 6-4 photoproducts(6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX(DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2)(also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8)(the CSA complex) plays a role in transcription-coupled repair(TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.

IMMUNOGEN INFORMATION

Immunogen

damage-specific DNA binding protein 1, 127kDa

Synonyms

DDB p127 subunit, DDB1, DDBA, DNA damage binding protein 1, DNA damage binding protein a, HBV X associated protein 1, UV damaged DNA binding factor, UV DDB 1, UV

Observed MW

127 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:200-1:800

UNIPROT INFORMATION

UniProt ID

Q16531

IMAGES