anti- DDX21 antibody

The anti-DDX21 antibody, available in a 100µg size, is a valuable tool for researchers investigating the functions of DEAD-box RNA helicase 21 (DDX21) in RNA metabolism and cellular processes. DDX21 is an essential RNA helicase involved in RNA unwinding and ribosome biogenesis.

This antibody is meticulously designed to specifically target DDX21 with exceptional specificity and sensitivity, making it suitable for various applications, including Western blotting, immunoprecipitation, and RNA-binding assays.

Our anti-DDX21 antibody is sourced from high-quality materials and undergoes rigorous validation to ensure reliable and reproducible results. It is a trusted choice for researchers studying RNA helicases, ribosomal RNA processing, and the regulation of gene expression.

With a 100µg size, this antibody provides ample supply for multiple experiments and is compatible with various sample types, including cell lysates and RNA-protein complexes. Whether exploring ribosome assembly, RNA unwinding, or the role of DDX21 in disease mechanisms, this antibody is an indispensable resource for your research.

Product Name

DDX21 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase(Pol) I and II: promotes ribosomal RNA(rRNA) processing and transcription from polymerase II(Pol II)(PubMed:25470060). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs(PubMed:25470060). In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes(PubMed:25470060, PubMed:25477391). In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes(PubMed:25470060). Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77'(PubMed:11823437, PubMed:25260534). Can unwind double-stranded RNA(helicase) and can fold or introduce a secondary structure to a single-stranded RNA(foldase)(PubMed:9461305). Involved in rRNA processing(PubMed:14559904, PubMed:18180292).

IMMUNOGEN INFORMATION

Immunogen

DEAD(Asp-Glu-Ala-Asp) box polypeptide 21

Observed MW

87 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q9NR30

IMAGES