anti- DGCR8 antibody

The anti-DGCR8 antibody, available in a 100µg size, is a valuable tool for researchers studying DGCR8 (DiGeorge Syndrome Critical Region 8), a key component of the microRNA biogenesis pathway, critical for the production of small regulatory RNAs.

This antibody is meticulously designed to specifically target DGCR8 with exceptional specificity and sensitivity, making it ideal for applications such as Western blotting, immunohistochemistry, and studies related to microRNA biogenesis, RNA processing, and gene regulation.

Our anti-DGCR8 antibody is sourced from high-quality materials and undergoes rigorous validation to ensure reliable and reproducible results. Researchers investigating the roles of DGCR8 in small RNA processing, cellular differentiation, or its implications in developmental disorders will find this antibody indispensable for their work.

With a 100µg size, each vial provides ample supply for multiple experiments, allowing for flexibility in experimental design. Whether unraveling DGCR8's function in microRNA-mediated gene regulation, embryonic development, or its involvement in congenital syndromes, this antibody is an essential resource for your research.

Product Name

DGCR8 antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Component of the microprocessor complex that acts as a RNA-and heme-binding protein that is involved in the initial step of microRNA(miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts(pri-miRNAs) to release precursor miRNA(pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs(PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer(of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding(PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine(m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing(PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal(By similarity).

IMMUNOGEN INFORMATION

Immunogen

DiGeorge syndrome critical region gene 8

Synonyms

C22orf12, DGCRK6

Observed MW

120 kDa

APPLICATION

Tested Application

ELISA, WB, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:1000

UNIPROT INFORMATION

UniProt ID

Q8WYQ5

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