anti- DHFR antibody

The anti-DHFR antibody, available in a 100µg size, is an essential tool for researchers studying the DHFR (Dihydrofolate Reductase) enzyme, a key player in folate metabolism and a target for antimicrobial and anticancer drugs.

This antibody is meticulously designed to specifically target DHFR with exceptional specificity and sensitivity, making it ideal for applications such as Western blotting, immunohistochemistry, and studies related to cancer biology, drug development, and folate metabolism.

Our anti-DHFR antibody is sourced from high-quality materials and undergoes rigorous validation to ensure reliable and reproducible results. Researchers investigating the molecular mechanisms of folate metabolism, drug resistance, or the role of DHFR in cellular proliferation will find this antibody indispensable for their work.

With a 100µg size, each vial provides ample supply for multiple experiments, allowing for flexibility in experimental design. Whether exploring DHFR's function in cancer therapy, antimicrobial drug resistance, or its implications in metabolic disorders, this antibody is an essential resource for your research.

Product Name

DHFR antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.

IMMUNOGEN INFORMATION

Immunogen

dihydrofolate reductase

Synonyms

DHFR, DHFRP1, dihydrofolate reductase

Observed MW

21 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP, IF

Recommended Dilution

WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF： 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P00374

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab02365(DHFR Antibody) at dilution of 1:50

Immunofluorescent analysis of HeLa cells using FNab02365 (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

IP Result of anti-DHFR (IP: FNab02365, 3ug; Detection: FNab02365 1:500) with Raji cells lysate 2000ug

K-562 cells were subjected to SDS PAGE followed by western blot with FNab02365(DHFR antibody) at dilution of 1:500