anti- DNMT1 antibody

Dive into the intricate world of epigenetics and DNA methylation with our Anti-DNMT1 Polyclonal Antibody, now available in a convenient 100µg size. DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) is a pivotal enzyme responsible for maintaining DNA methylation patterns during DNA replication.

This polyclonal antibody offers outstanding specificity and sensitivity, ensuring the precise detection of DNMT1 protein in your experiments. Researchers exploring the epigenome, DNA methylation, or related fields will greatly benefit from the accuracy and dependability that this antibody provides.

Our high-quality Anti-DNMT1 Polyclonal Antibody undergoes stringent quality control to ensure consistent and reproducible results, enabling you to advance your research with confidence. Investigate the dynamic world of DNA methylation and the vital role DNMT1 plays in maintaining epigenetic information. Whether you are researching DNA methylation patterns in development, disease, or other contexts, this antibody is an indispensable tool for making significant strides in understanding these fundamental processes.

Product Name

DNMT1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes(TSGs) or other tumor-related genes in colorectal cancer(CRC) cells(PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells(ESCs)(PubMed:24623306). Associates at promoter regions of tumor suppressor genes(TSGs) leading to their gene silencing(PubMed:24623306). Promotes tumor growth(PubMed:24623306).

IMMUNOGEN INFORMATION

Immunogen

DNA(cytosine-5-)-methyltransferase 1

Synonyms

AIM, CXXC9, DNA MTase HsaI, DNMT, DNMT1, MCMT

Observed MW

180-200 kDa

APPLICATION

Tested Application

ELISA, IHC, IP, WB, IF

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

P26358

IMAGES