anti- EIF4A3 antibody

Accelerate your RNA research with our Anti-EIF4A3 Antibody, now available in a convenient 100µg size. EIF4A3 (Eukaryotic Translation Initiation Factor 4A3) is a pivotal player in the initiation of translation, RNA processing, and mRNA surveillance. It is an essential component of exon junction complexes (EJCs), contributing to various aspects of RNA metabolism.

This antibody is meticulously validated for specificity, ensuring that your investigations into EIF4A3's role in RNA splicing, nonsense-mediated mRNA decay (NMD), and mRNA transport are fortified by a reliable tool. Whether you're deciphering the intricacies of RNA regulation, alternative splicing, or mRNA quality control, this antibody is an indispensable addition to your research arsenal.

Order your 100µg vial today to unlock the potential of EIF4A3 and its multifaceted functions in RNA biology.

Product Name

EIF4A3 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex(EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay(NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X(and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. Involved in craniofacial development.

IMMUNOGEN INFORMATION

Immunogen

eukaryotic translation initiation factor 4A, isoform 3

Synonyms

DDX48, KIAA0111

Observed MW

47 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P38919

IMAGES