anti- FOXO3A antibody

Investigate the functions of Forkhead Box O3A (FOXO3A) with precision using our Anti-FOXO3A Antibody, available in two 100µg vials. FOXO3A is a transcription factor with essential roles in cellular processes, including apoptosis and autophagy. This highly specific polyclonal antibody empowers researchers to delve into FOXO3A's functions, contributing to a deeper understanding of its impact on cell biology and health.

Key Features:

• Exceptional Specificity: Our Anti-FOXO3A Antibody is rigorously tested to ensure it accurately targets FOXO3A, minimizing the risk of cross-reactivity.
• Ample Quantity: With two 100µg vials, you have a generous supply for multiple experiments.
• Versatile Applications: Ideal for various experimental techniques, including immunohistochemistry, Western blotting, and ChIP assays.
• Top-Tier Quality: Manufactured to the highest industry standards, guaranteeing consistent and reproducible results.
• Advance Your Research: Whether you're studying apoptosis, autophagy, or FOXO3A functions, our Anti-FOXO3A Antibody is your precise tool for FOXO3A research.

Product Name

FOXO3A antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

Forkhead box O-class (FOXO) transcription factors are mammalian homologue of DAF-16, a known lifespan regulator of Caenorhabditis elegans. FOXO transcription factors, such as FOXO1, FOXO3a, and FOXO4, have been linked to the regulation of cell cycle, apoptosis, DNA repair, oxidative stress, and metabolism. FOXO proteins are proposed to be dual regulated by Akt-mediated phosphorylation and Skp2-mediated ubiquitination, which are usually initiated by growth factors and cytokines. FOXO proteins are also activated by stress, which induces gene expression that contributes to cell cycle arrest, implicating FOXO proteins as tumor suppressors. Specifically, FOXO3a (FKHR-L1) is a transcriptional activator for apoptosis in the absence of survival factors, such as in neural cell death triggered by oxidative stress. In the presence of survival factors like IGF-1, AKT1/PKB phosphorylates on Thr-32 and Ser-253. FOXO3a is inactivated when phosphorylated by AKT1 on Thr-32 and Ser-253.

IMMUNOGEN INFORMATION

Immunogen

forkhead box O3

Synonyms

AF6q21, AF6q21 protein, DKFZp781A0677, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A

Observed MW

90 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200

UNIPROT INFORMATION

UniProt ID

O43524

IMAGES