anti- GADD34 antibody

Embark on an exploration of cellular stress responses with our Anti-GADD34 Antibody, available in a 100µg size. GADD34 (Growth Arrest and DNA Damage-Inducible Protein 34) plays a crucial role in the unfolded protein response, regulating cellular adaptation to endoplasmic reticulum stress. This antibody is meticulously designed to specifically target GADD34, enabling you to delve into its functions with precision.

Key Features:

• Precise Targeting: Rigorously validated to exclusively recognize GADD34, reducing the risk of non-specific binding to other proteins.
• Versatile Applications: Ideal for various experimental techniques, including Western blotting, immunofluorescence, and immunoprecipitation, empowering your research in endoplasmic reticulum stress and stress signaling pathways.
• Adequate Quantity: With 100µg of antibody, you have enough material for multiple experiments, reducing the need for frequent restocking.
• Quality Guaranteed: Manufactured under stringent quality control measures to ensure the consistency and reliability of your results.
• Uncover Stress Signaling Mechanisms: Whether you're investigating GADD34's role in protein homeostasis, unfolded protein response, or potential therapeutic targets for diseases like Alzheimer's, our Anti-GADD34 Antibody is your indispensable tool.

• Product Name

  GADD34 antibody

  Size

  100µg

  Form

  liquid

  Purification

  Immunogen affinity purified

  Purity

  ≥95% as determined by SDS-PAGE

  Host

  Rabbit

  Clonality

  polyclonal

  Isotype

  IgG

  Storage

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

  BACKGROUND

  GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4(ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase(PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 appears to be about 100 kDa which was confirmed in GADD34 KO mice and drug treated cells, despite the predicated MW of 73 kDa, and this antibody primarily recongize the 100 kDa band.

  IMMUNOGEN INFORMATION

  Immunogen

  protein phosphatase 1, regulatory(inhibitor) subunit 15A

  Synonyms

  GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A

  Observed MW

  100 kDa

  APPLICATION

  Tested Application

  ELISA, WB, IHC, IF

  Recommended Dilution

  WB: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

  UNIPROT INFORMATION

  UniProt ID

  O75807

  IMAGES

   

  

  Immunohistochemistry of paraffin-embedded human pancreas cancer using FNab03293(GADD34 antibody) at dilution of 1:100

  

  Immunofluorescent analysis of PC-3 cells using FNab03293( GADD34 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

  

  K-562 cells were subjected to SDS PAGE followed by western blot with FNab03293(GADD34 antibody) at dilution of 1:600