anti- GALE antibody

Unveil the mysteries of GALE (Galactose Epimerase) and its role in galactose metabolism with our Anti-GALE Antibody, available in a 100µg size. GALE is a vital enzyme that plays a key role in converting galactose to glucose, and its dysregulation is associated with various metabolic disorders. This antibody is expertly crafted to specifically target GALE, enabling you to study its functions with precision.

Key Features:

• Exceptional Specificity: Thoroughly validated to exclusively recognize GALE, minimizing the potential for cross-reactivity with other proteins.
• Versatile Applications: Ideal for various experimental techniques, including Western blotting, enzyme assays, and immunohistochemistry, empowering your research in carbohydrate metabolism and metabolic diseases.
• Adequate Quantity: With 100µg of antibody, you have enough material for multiple experiments, reducing the need for frequent restocking.
• Quality Guaranteed: Manufactured under strict quality control measures to ensure the consistency and reliability of your results.
• Explore Metabolic Pathways: Whether you're investigating GALE's role in galactosemia, liver disease, or metabolic syndromes, our Anti-GALE Antibody is your indispensable tool.

• Product Name

  GALE antibody

  Size

  100µg

  Form

  liquid

  Purification

  Immunogen affinity purified

  Purity

  ≥95% as determined by SDS-PAGE

  Host

  Rabbit

  Clonality

  polyclonal

  Isotype

  IgG

  Storage

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

  BACKGROUND

  This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified.

  IMMUNOGEN INFORMATION

  Immunogen

  UDP-galactose-4-epimerase

  Observed MW

  36 kDa

  APPLICATION

  Tested Application

  ELISA, WB, IHC, IF

  Recommended Dilution

  WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:10 - 1:100

  UNIPROT INFORMATION

  UniProt ID

  Q14376

  IMAGES

   

  

  Immunohistochemical of paraffin-embedded human malignant melanoma using FNab03309(GALE antibody) at dilution of 1:100

  

  Immunofluorescence analysis of A549 cells using FNab03309(GALE antibody) at dilution of 1:50

  

  A375 cells were subjected to SDS PAGE followed by western blot with FNab03309(GALE antibody) at dilution of 1:1000