anti- GFP tag antibody

Delve into the world of molecular biology and protein expression with our Anti-GFP Tag Monoclonal Antibody. This carefully crafted antibody is your key to detecting and studying proteins fused with the Green Fluorescent Protein (GFP) tag. Key features include:

• Monoclonal Advantage: This antibody is a monoclonal, providing high specificity and a lower risk of cross-reactivity, ensuring precise and accurate results in your experiments.

• Wide Applications: Ideal for various research methodologies, including Western blotting, immunoprecipitation, and immunofluorescence, this antibody is a valuable addition to your molecular biology toolkit.

• Consistent Results: Whether you are working with cell cultures, tissues, or model organisms, this antibody offers consistent and reproducible results, making it a dependable choice for your experiments.

• 100µg Size: The 100µg size ensures ample supply for multiple experiments, allowing you to focus on your research without concerns of running out of reagents.

Unleash the power of precision in detecting GFP-tagged proteins with our Anti-GFP Tag Monoclonal Antibody, and confidently advance your research in molecular and cell biology.

Product Name

GFP tag antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP. Note: Do not add Azium(Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.

IMMUNOGEN INFORMATION

Immunogen

GFP tag

Synonyms

CFP, eGFP, eYFP, GFP, GFP tag, YFP

Observed MW

30 kDa

APPLICATION

Tested Application

ELISA, WB, IP, IF

Recommended Dilution

WB: 1:2000-1:16000; IP: 1:1000-1:4000; IF: 1:20-1:200

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