anti- GPR177 antibody

anti- GPR177 antibody

Product Name

GPR177 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).

IMMUNOGEN INFORMATION

Immunogen

G protein-coupled receptor 177

Synonyms

C1orf139, EVI, G protein coupled receptor 177, GPR177, Protein wntless homolog, Sprinter, WLS, Wntless

Observed MW

46-62 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q5T9L3

IMAGES

Immunohistochemistry of paraffin-embedded human ovary using FNab03601(GPR177 antibody) at dilution of 1:100

IP Result of anti-GPR177 (IP: FNab03601, 3ug; Detection: FNab03601 1:500) with mouse liver tissue lysate 10000ug.

mouse liver tissue were subjected to SDS PAGE followed by western blot with FNab03601(GPR177 antibody) at dilution of 1:500