anti- GRP75 antibody

anti- GRP75 antibody

Product Name

GRP75 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

GRP75(also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA(HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson’s disease.

IMMUNOGEN INFORMATION

Immunogen

heat shock 70kDa protein 9(mortalin)

Synonyms

CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74

Observed MW

74 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, FC, IP

Recommended Dilution

WB: 1:1000-1:4000; IP: 1:1000-1:4000; IHC: 1:20-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

P38646

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer using FNab03661(GRP75 antibody) at dilution of 1:100

Immunofluorescent analysis of HepG2 cells using FNab03661 (GRP75 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

IP Result of anti-GRP75 (IP:FNab03661, 4ug; Detection:FNab03661 1:2000) with mouse brain tissue lysate 3680ug.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab03661(GRP75 antibody) at dilution of 1:2000