anti- GSK3B antibody

anti- GSK3B antibody

Product Name

GSK3B antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Glycogen synthase kinase-3(GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the C-terminal of GSK3B.

IMMUNOGEN INFORMATION

Immunogen

glycogen synthase kinase 3 beta

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta

Observed MW

50 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

P49841

IMAGES

Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab03676(GSK3B Antibody) at dilution of 1:50

Immunofluorescent analysis of HeLa cells using FNab03676 (GSK3B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

IP Result of anti-GSK3B (IP: FNab03676, 3ug; Detection: FNab03676 1:500) with HeLa cells lysate 800ug.

mouse ovary tissue were subjected to SDS PAGE followed by western blot with FNab03676(GSK3B antibody) at dilution of 1:1000