anti- GST tag antibody

Product Name

GST tag antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2a

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein.

IMMUNOGEN INFORMATION

Immunogen

Recombinant protein

Synonyms

Glutathione S-Transferase, GST, gst tag

APPLICATION

Tested Application

ELISA, WB, IP

Recommended Dilution

WB: 1:5000-1:50000; IP: 1:5000-1:20000

IMAGES

IP result of anti-GST Tag(IP:FNab03684, 5ug; Detection:FNab03684 1:10000) with Total E. coli lysate (with pGEX4T fused plasmid) 1360 ug

GST tagged recombinant protein(25ng/lane) was subjected to SDS-PAGE followed by western blot with anti-GST tag monoclonal antibody FNab03684 at dilution of 1:40000