anti- HER2/ErbB2 antibody

anti- HER2/ErbB2 antibody

Product Name

HER2/ErbB2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor(EGF) receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas. This antibody raised against a synthesized peptide corresponding to 1237-1255aa of human HER2 recognizes the 185-kDa full-length glycosylated form and other lower molecular-mass forms of HER2, including the truncated form.

IMMUNOGEN INFORMATION

Immunogen

v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog(avian)

Synonyms

CD340, ERBB2, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1

Observed MW

185 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, FC, IP

Recommended Dilution

WB: 1:1000-1:10000; IP: 1:200-1:2000; IHC: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

P04626

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer using FNab03833(ERBB2 antibody) at dilution of 1:50

IP Result of anti-HER2/ErbB2 (IP:FNab03833, 5ug; Detection:FNab03833 1:500) with SGC-7901 cells lysate 2400ug.

HeLa cells were subjected to SDS PAGE followed by western blot with FNab03833(ERBB2 antibody) at dilution of 1:300