anti- HER2/ErbB2 antibody

anti- HER2/ErbB2 antibody

Product Name

HER2/ErbB2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors.

IMMUNOGEN INFORMATION

Immunogen

v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog(avian)

Synonyms

CD340, ERBB2, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1

Observed MW

185 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P04626

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer using FNab03834(ERBB2 antibody) at dilution of 1:50

Immunofluorescent analysis of HeLa cells using FNab03834 (ErbB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

HeLa cells were subjected to SDS PAGE followed by western blot with FNab03834(ERBB2 antibody) at dilution of 1:500