anti- HGS antibody

anti- HGS antibody

Product Name

HGS antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body(MVBs) when complexed with STAM(ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.

IMMUNOGEN INFORMATION

Immunogen

hepatocyte growth factor-regulated tyrosine kinase substrate

Synonyms

HRS

Observed MW

110-115 kDa

APPLICATION

Tested Application

ELISA, WB, IP, IF

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

O14964

IMAGES

IP Result of anti-HGS (IP:FNab03853, 5ug; Detection:FNab03853 1:1000) with mouse brain tissue lysate 3000ug.

HeLa cells were subjected to SDS PAGE followed by western blot with FNab03853(HGS antibody) at dilution of 1:800