anti- Histone H1.2 antibody

anti- Histone H1.2 antibody

Product Name

Histone H1.2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Histone H1 regulates chromatin competency through its high affinity binding to linker DNA as a structural component. It acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. The calcualted molecular weight of Histone H1.2 is 21 kDa, but modified Histone H1.2 is about 32 kDa.

IMMUNOGEN INFORMATION

Immunogen

histone cluster 1, H1c

Synonyms

H1.2, H1F2, HIST1H1C, histone cluster 1, H1c, Histone H1.2, Histone H1d

Observed MW

32kd

APPLICATION

Tested Application

ELISA, IHC, WB, IF, IP

Recommended Dilution

WB: 1:500-1:3000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

P16403

IMAGES

Immunohistochemical of paraffin-embedded human ovary tumor using FNab03883(HIST1H1C antibody) at dilution of 1:100

Immunofluorescent analysis of Hela cells, using HIST1H1C antibody FNab03883 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-Histone H1.2 (IP: FNab03883, 3ug; Detection: FNab03883 1:600) with HeLa cells lysate 1200ug.

L02 cells were subjected to SDS PAGE followed by western blot with FNab03883(HIST1H1C antibody) at dilution of 1:500