anti- Histone H2A.X antibody

anti- Histone H2A.X antibody

Product Name

Histone H2A.X antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

IMMUNOGEN INFORMATION

Immunogen

H2A histone family, member X

Synonyms

H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x

Observed MW

16 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:100 - 1:200; IF: 1:50 - 1:200

UNIPROT INFORMATION

UniProt ID

P16104

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer using FNab03885(H2AFX antibody) at dilution of 1:100

HL-60 cells were subjected to SDS PAGE followed by western blot with FNab03885(H2AFX antibody) at dilution of 1:1000