anti- Histone-H3 antibody

anti- Histone-H3 antibody

Product Name

Histone-H3 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

HIST2H3A,histone cluster 2, H3a.It is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. HIST2H3A is Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

IMMUNOGEN INFORMATION

Immunogen

histone cluster 2, H3a

Synonyms

H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o

Observed MW

15-17 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500-1:5000; IHC: 1:50-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q71DI3

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab03890(Histone-H3 Antibody) at dilution of 1:50

Immunofluorescent analysis of HEK-293 cells using FNab03890 (Histone-H3 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG

MCF7 cells were subjected to SDS PAGE followed by western blot with FNab03890(Histone-H3 antibody) at dilution of 1:1000