anti- HLA class I(HLA-A) antibody

anti- HLA class I(HLA-A) antibody

Product Name

HLA class I(HLA-A) antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

IMMUNOGEN INFORMATION

Immunogen

major histocompatibility complex, class I, A

Synonyms

HLA A, HLAA, HLA-A

Observed MW

44 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

P04439/P30443

IMAGES

Immunohistochemistry of paraffin-embedded human esophagus using FNab03895(HLA class I(HLA-A) antibody) at dilution of 1:100

HepG2 cells were subjected to SDS PAGE followed by western blot with FNab03895(HLA class I(HLA-A) antibody) at dilution of 1:1000