anti- HMGB1 antibody

anti- HMGB1 antibody

Product Name

HMGB1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

IMMUNOGEN INFORMATION

Immunogen

high-mobility group box 1

Synonyms

DKFZp686A04236, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

Observed MW

26 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200

UNIPROT INFORMATION

UniProt ID

P09429

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer using FNab03924(HMGB1 antibody) at dilution of 1:100

Immunofluorescence analysis of HeLa cells using FNab03924 (HMGB1 antibody) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab03924(HMGB1 antibody) at dilution of 1:1000