anti- HMGCR antibody

anti- HMGCR antibody

Product Name

HMGCR antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

IMMUNOGEN INFORMATION

Immunogen

3-hydroxy-3-methylglutaryl-Coenzyme A reductase

Synonyms

HMG CoA reductase, HMGCR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase, HMDH

Observed MW

100 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200

UNIPROT INFORMATION

UniProt ID

P04035

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer using FNab03929(HMGCR antibody) at dilution of 1:100

HeLa cells were subjected to SDS PAGE followed by western blot with FNab03929(HMGCR antibody) at dilution of 1:1000