anti- HNRNPD antibody

anti- HNRNPD antibody

Product Name

HNRNPD antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Binds with high affinity to RNA molecules that contain AU-rich elements(AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double-and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability(mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation.

IMMUNOGEN INFORMATION

Immunogen

heterogeneous nuclear ribonucleoprotein D(AU-rich element RNA binding protein 1, 37kDa)

Synonyms

AUF1;AUF1A;HNRPD;P37;hnRNPD0

Observed MW

40 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:100

UNIPROT INFORMATION

UniProt ID

Q14103

IMAGES

Immunohistochemistry of paraffin-embedded human cervical cancer using FNab03950(HNRNPD antibody) at dilution of 1:100

Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using FNab03950 (HNRNPD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

HeLa cells were subjected to SDS PAGE followed by western blot with FNab03950(HNRNPD antibody) at dilution of 1:500