anti- HNRNPK antibody

anti- HNRNPK antibody

Product Name

HNRNPK antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction(By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21(lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.

IMMUNOGEN INFORMATION

Immunogen

heterogeneous nuclear ribonucleoprotein K

Synonyms

CSBP, hnRNP K, HNRNPK, HNRPK, TUNP

Observed MW

51 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, FC

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

P61978

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer using FNab03955(HNRNPK antibody) at dilution of 1:50

Immunofluorescent analysis of HepG2 cells using FNab03955(HNRNPK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

HeLa cells were subjected to SDS PAGE followed by western blot with FNab03955(HNRNPK antibody) at dilution of 1:300