anti- HPSE antibody

anti- HPSE antibody

Product Name

HPSE antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Endoglycosidase that cleaves heparan sulfate proteoglycans(HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix(ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix(ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.

IMMUNOGEN INFORMATION

Immunogen

heparanase

Synonyms

HEP, HPA, HPA1, HPR1, HPSE1, HSE1

Observed MW

60 kDa

APPLICATION

Tested Application

ELISA, IHC, WB

Recommended Dilution

WB: 1:200-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q9Y251

IMAGES

Immunohistochemistry of paraffin-embedded human liver cancer using FNab04003(HPSE antibody) at dilution of 1:50

DU 145 cells were subjected to SDS PAGE followed by western blot with FNab04003(HPSE antibody) at dilution of 1:300