anti- HTRA1 antibody

anti- HTRA1 antibody

Product Name

HTRA1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors(IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.

IMMUNOGEN INFORMATION

Immunogen

HtrA serine peptidase 1

Synonyms

HTRA, PRSS11

Observed MW

35 kDa, 50 kD, 42 kDa

APPLICATION

Tested Application

ELISA, WB, IF, IP, IHC

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q92743

IMAGES

Immunohistochemistry of paraffin-embedded human placenta tissue slide using FNab04069(HTRA1 Antibody) at dilution of 1:200

IP Result of anti-HTRA1 (IP:FNab04069, 4ug; Detection:FNab04069 1:500) with L02 cells lysate 1400ug.

L02 cells were subjected to SDS PAGE followed by western blot with FNab04069(HTRA1 antibody) at dilution of 1:600