anti- Lamin B1 antibody

anti- Lamin B1 antibody

Product Name

Lamin B1 antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2a

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

IMMUNOGEN INFORMATION

Immunogen

lamin B1

Synonyms

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

Observed MW

66kd

APPLICATION

Tested Application

ELISA, WB, IF, FC, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:5000-1:50000 ; IHC: 1:500-1:2000; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P20700

IMAGES

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using FNab04683(Lamin B1 antibody) at dilution of 1:1000

IP Result of anti-Lamin B1 (IP: FNab04683, 4ug; Detection: FNab04683 1:40000) with HeLa cells lysate 2000ug.

HeLa cells were subjected to SDS PAGE followed by western blot with FNab04683(LMNB1 antibody) at dilution of 1:10000