anti- LC3 antibody

anti- LC3 antibody

Product Name

LC3 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.1% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Map1LC3, also known as LC3, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18-kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 16-kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

IMMUNOGEN INFORMATION

Immunogen

microtubule-associated protein 1 light chain 3 beta

Synonyms

LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B

Observed MW

16 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:2000 ; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q9GZQ8

IMAGES

Immunohistochemistry of paraffin-embedded human gliomas using FNab04717(LC3 antibody) at dilution of 1:50

IP Result of anti-LC3 (IP: FNab04717, 3ug; Detection: FNab04717 1:1000) with mouse brain tissue lysate 4000ug.

Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with FNab04717(LC3 Antibody) at dilution of 1:1000