anti- LEF1 antibody

anti- LEF1 antibody

Product Name

LEF1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1(By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

IMMUNOGEN INFORMATION

Immunogen

lymphoid enhancer-binding factor 1

Observed MW

40-45 kDa

APPLICATION

Tested Application

ELISA, IHC, WB, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q9UJU2

IMAGES

Immunohistochemistry of paraffin-embedded human thymus using FNab04745(LEF1 antibody) at dilution of 1:100

IP Result of anti-LEF1 (IP:FNab04745, 4ug; Detection:FNab04745 1:300) with SW 1990 cells lysate 2400ug.

COLO 320 cells were subjected to SDS PAGE followed by western blot with FNab04745(LEF1 Antibody) at dilution of 1:300