anti- LIG4 antibody

anti- LIG4 antibody

Product Name

LIG4 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining(NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

IMMUNOGEN INFORMATION

Immunogen

ligase IV, DNA, ATP-dependent

Observed MW

100-104 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P49917

IMAGES

Immunohistochemistry of paraffin-embedded human prostate cancer using FNab04774(LIG4 antibody) at dilution of 1:100

mouse testis tissue were subjected to SDS PAGE followed by western blot with FNab04774(LIG4 antibody) at dilution of 1:600