anti- LIN28B antibody

anti- LIN28B antibody

Product Name

LIN28B antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Suppressor of microRNA(miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts(pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA(PubMed:22118463). Does not act on pri-miR21(PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation(PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent(PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression(By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

IMMUNOGEN INFORMATION

Immunogen

lin-28 homolog B(C. elegans)

Synonyms

CSDD2

Observed MW

27 kDa

APPLICATION

Tested Application

ELISA, WB

Recommended Dilution

WB: 1:500-1:2000

UNIPROT INFORMATION

UniProt ID

Q6ZN17

IMAGES

mouse embryo tissue were subjected to SDS PAGE followed by western blot with FNab04785(LIN28B antibody) at dilution of 1:300