anti- MAF antibody

anti- MAF antibody

Product Name

MAF antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2(Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE(Maf response element) sites of lens-specific alpha-and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif(atypical Maf response element) in the CD13 proximal promoter in endothelial cells(By similarity). When overexpressed, represses anti-oxidant response element(ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.

IMMUNOGEN INFORMATION

Immunogen

v-maf musculoaponeurotic fibrosarcoma oncogene homolog

Synonyms

c MAF, cMAF, MAF, Proto oncogene c Maf, Transcription factor Maf

Observed MW

50 kDa

APPLICATION

Tested Application

ELISA, IHC, WB, IP,IF

Recommended Dilution

WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

O75444

IMAGES

Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab04929(MAF Antibody) at dilution of 1:50

Immunofluorescent analysis of A431 cells using FNab04929(MAF antibody) at dilution of 1:50

IP Result of MAF antibody(IP: FNab04929, 4μg ; Detection: FNab04929 1:500) with A431 cell lysate 2000μg.

A431 cells were subjected to SDS PAGE followed by western blot with FNab04929(MAF antibody) at dilution of 1:500