anti- MAGOH antibody

anti- MAGOH antibody

Product Name

MAGOH antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Core component of the splicing-dependent multiprotein exon junction complex(EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay(NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X(and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.

IMMUNOGEN INFORMATION

Immunogen

mago-nashi homolog, proliferation-associated(Drosophila)

Synonyms

MAGOHA

Observed MW

17 kDa

APPLICATION

Tested Application

ELISA, IHC, IP, WB

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P61326

IMAGES

Immunohistochemistry of paraffin-embedded human ovary tumor using FNab04954(MAGOH antibody) at dilution of 1:50

IP Result of anti-MAGOH (IP:FNab04954, 3ug; Detection:FNab04954 1:500) with K-562 cells lysate 2400ug.

K-562 cells were subjected to SDS PAGE followed by western blot with FNab04954(MAGOH Antibody) at dilution of 1:1000

RELATED PRODUCTS

• Recombinant Human MAGOH
• Recombinant Human Mago nashi homolog 2

STATEMENT

Manuals on the web are for your reference only, specifications are subject to the delivery manuals.

FEATURED PRODUCT